...Thiocyanate elution; Enzyme linked immunosorbent assay; Phage display technique [gap=765]关键词: 人源化; 单链抗体; 组合抗体库; 肝癌; 重叠延伸PCR; 硫氰酸盐洗脱法; 酶联免疫吸附试验; 噬菌体展示技术 ..
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Objective To establish a method for the measurement of relative affinity of phage antibodies. Methods The relative affinities of 5 phage antibodies were measured by ELISA using thiocyanate elution.
亲和力是反映抗体特性的一个重要指标,也是抗体研究中经常需要测定的参数,在抗体的筛选、鉴定乃至其功能和应用研究等方面都涉及到亲和力的问题。
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